Journal: bioRxiv

Article Title: A novel fracture lattice in spiny mouse skin facilitates tissue autotomy and regeneration

doi: 10.64898/2026.03.23.713756

Figure Lengend Snippet: Genetic and pharmacologic manipulations for inhibiting spiny hair formation. a , H&E images of horizontal sections of Acomys back skin at different developmental stages. Note that during early development, the fracture lattice is absent. Scale bars, 1 mm. b , Schematic illustrations depicting the remodeling process of the fracture lattice from a primitive pattern (P21) to a definitive pattern (P77). c , Schematic depicting the design of lentiviral construct for overexpression Acomys Dkk1 gene. Acomys DKK1 expression and secretion were confirmed by Western blot analysis using an anti-Myc-tag antibody. d , (left) Representative photo of ultrasound-guided lentivirus injection into the yolk sac cavity of an E16.5 Acomys embryo. (right) Fluorescence stereoscope image showing strong expression of H2B-RFP in an E30.5 Acomys embryo. e , Representative images showing that overexpression of AcoDKK1 effectively blocked the formation of hair follicles in Acomys skin. Scale bars, 200μm. f , Experimental timeline of AcoDKK1 overexpression and analysis of fracture lattice formation. g , Experimental timeline of 5-FU treatment and analysis of fracture lattice formation. h , Photographs showing that topical 5-FU treatment effectively inhibited spiny hair formation in Acomys back skin.

Article Snippet: After blocking with 5% skim milk in TBST solution (1X TBS, 0.1% Tween-20) for 1 h at room temperature, the membrane was incubated with mouse anti-Myc-Tag (1:1000, Cell Signaling Cat# 2276S) at 4°C overnight and HRP-conjugated anti-mouse IgG secondary antibody (1:1000, Jackson ImmunoResearch).

Techniques: Construct, Over Expression, Expressing, Western Blot, Injection, Fluorescence